Chicken Egg Yolk Antibodies

The presence of anti-human egg yolk antibody (AEA), the IgG antibodies to the crude egg yolk, was measured using indirect enzyme-linked immunoassay (ELISA). The negative cut-off point for AEA was greater than 97.5 U/mL.

In group 1, antibodies to the crude egg yolk were present in 1 of the 6, and no antibodies were found in the second, and in the 3rd blood sample. The mean absorbance ratio for the E~492~ (signal of the antibody) to the signals for IgG (baseline signal) for anti-egg yolk antibodies were 0.0343+/-0.0229 and for the IgG1 anti-egg yolk antibodies-0.0618+/-0.0538, and the difference between the two ratios was significant.

In group 2, antibodies to crude egg yolk were present in 1 of 6, and antibodies were found in the 2nd and 3rd blood samples. The mean absorbance ratio was 0.0118+/-0.0377 for E~492~/IgG1. There was a statistically significant difference between the E~492~ and IgG1 absorbance ratios.

The mean absorbance ratio for the E~492~/IgG1 was 1.7087+/-0.1917 in group 1; IgG1/IgG1 was 0.0152+/-0.0336. Comparison of the mean Absorbance Ratio IgG1/IgG1 vs E~492~/IgG1 shows a significant difference (P value = 0.039).

It can also be inferred from [Table 3](#T3){ref-type="table"} that in all 7 patients, the mean absorbance ratio for the E~492~/IgG1 (IgG1/IgG1) was significantly less than that for all the other parameters (Absorbance Ratio for F~492~/F~492~, F~492~/E~492~, F~492~/I~492~, E~492~/I~492~, F~492~/I~492~ and E~492~/I~492~).